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Proteomic studies of human and mouse tubulin typically result in the analysis of a large number of proteins, many of which are specific isoforms. Their identification by mass spectrometry (MS) depends on efficient sample preparation and separation. Proteomic studies of tubulin have taken advantage of its interaction with F-actin and peptide microtubule stabilizing agents, leading to the identification of binding partners. One approach is based on chemical crosslinking of purified tubulin with glutaraldehyde. The resulting cross-linked polymers are purified by affinity chromatography using tubulin antibodies as a probe. Peptides resulting from the digestion of the cross-linked polymers are analysed by MS/MS fragmentation and results are matched to a database. The approach is powerful, but it also has several drawbacks. The use of antibodies to isolate crosslinked polymers is a major source of errors in the identification process, which depend on the specificity of the antibodies used and the type of proteins targeted. This is illustrated here by the analysis of the tubulin-F-actin microtubule cross-linking. We also review briefly two alternative approaches to affinity purification of tubulin complexes: chemical cross-linking and methods based on reversible chemical modification of the beta-tubulin thiols. Finally, we describe in more detail the use of LysC and Trypsin in the preparation of samples for MS/MS analysis.This is a great site for real-time traffic, news, and weather data for the greater Toronto area.
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How do I get a line start and end point from an area drawn with CGContextSetLineWidth?
I’m looking for a way to get the start and end points of a line that I’ve drawn using CGContextSetLineWidth
A:
I assume you’re trying to do something like this.
CGContextSetLineWidth(context, 5);
CGContextSetRGBFillColor(context, 0, 0, 0, 255);
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// Draw something.
CGContextDrawPath(context, kCGPathStroke);
// Then later draw some line from the previous
// point to the next
CGContextMoveToPoint(context, startPoint.x, startPoint.y);
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CGContextSetRGBStrokeColor(context, 0, 0, 0, 255);
CGContextSetLineWidth(context, 5);
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If you need to find the closest point in the line, I’d suggest you use a much better algorithm that uses a third party library, but the basic algorithm is something like this:
Find the end point using a standard point-in-polygon algorithm.
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