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The skin protects the body from many environmental stresses including ultraviolet (UV) radiation. In the present study, we have designed a novel, robust in vitro model of human skin to evaluate antioxidant and anti-cancer effects of various chemicals. Human skin was treated with a range of doses of α-phenyl-α-N-tosyl-N-hydroxysuccinimide ester (TOH), which increases skin sensitivity to UVA and UVB irradiation. This is followed by assessment of cell viability and inflammation using flow cytometry, reverse transcriptase-polymerase chain reaction and western blotting. The model was optimized using a panel of antioxidants. Specifically, vitamin E and β-carotene were effective antioxidants, and the assay was calibrated using the dose-response curves for each. We have identified a method to evaluate the effect of high-dose UVA on cultured human skin using a cytochrome P450 1A1 enzyme induction (CYP1A1) assay, an assay that will require further calibration. In the context of this study, high-dose UVA generated a higher reactive oxygen species (ROS) load than both UVA and UVB. However, using this in vitro model, TOH exposure failed to reduce the ROS load in keratinocytes and monocytes.Q:
Function overload not the same as class inheritance?
I’m trying to create a game of the mahjong tile-pushing game known as “Chinese Checkers”, and while I’ve got most of the basic framework in place I’m having a little bit of trouble with it’s initialisation.
The game has a Board class that contains all of the BoardGame’s variables and initialisation, and when the board is initialised it is passed as a parameter to the BoardGame’s init function. This function is located in a GameState class that contains the board variables.
// Board class
class Board {
… variables…
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