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The lethal effects of the divalent metal ions, Cu2+, Zn2+, Mn2+ and Co2+, on cells of the Gram-positive Bacillus subtilis and the Gram-negative Escherichia coli were studied. The membrane integrity of both types of cells was investigated by electron microscopy, analysis of membrane permeability, release of cytoplasmic constituents and membrane lipid composition. The bacterial cells were found to be significantly more sensitive to the metal ions than the yeasts. The results indicate that the total negative charge of the cell surface is the major determinant of the metal ion toxicity. E. coli cells were found to be more sensitive to the metal ions than B. subtilis cells. The differences in membrane lipid composition between the bacteria and the yeasts and the marked difference in sensitivity of the two cell types to the metal ions point to a close resemblance between their plasma membrane and the lipid bilayer, i.e., both belong to the so-called zwitterionic category. The lower susceptibility of the bacteria may be related to the greater proportion of negatively charged lipids of the bacterial plasma membrane, which is in contrast to the higher sensitivity of the yeasts.How does PostgreSQL use database replication?
Note that this only applies to PostgreSQL 8.0.3 and
above. PostgreSQL 7.4 and 7.5 used a
different protocol, and PostgreSQL 8.0.2 and 8.0.3 do not
have any special support for this.
Assuming your application cannot tolerate a loss of access to the
database, the PostgreSQL replication system solves the problem by
replicating the entire database on a slave. The network path
between master and slave has to be secure, so that the slave can
see all the data the master sees. However, the replica database
is identical to the master database, and the master can fail
with no side effects.
You must tell the PostgreSQL process to start
replication with the –replicate option, and tell
it to commit to the database file
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