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The different components of the bacteriophage T4 cell cycle were determined by RNA gel blot analysis. Asynchronous T4-infected cultures were examined at the times after infection when the host cells initiate DNA synthesis (about 45 min after infection), when DNA replication is inhibited by the presence of topoisomerase-I (about 90 min), and when DNA synthesis is inhibited by the product of the cI gene (late in infection). A new RNA size-fractionation procedure was used for the analysis of RNA isolated from the cells. A standard RNA concentration of 5 micrograms/ml was used. The different RNA species within each size fraction were resolved on RNA gel blots. The levels of host RNAs in the different fractions showed a complex series of temporal changes during the cell cycle. In contrast, the T4 RNA species were present in low amounts and were not detected until 45 min after infection. The T4 RNAs were present in all size fractions, indicating that they were either free in the cytoplasm or associated with the cell membrane. As expected, the level of the 5S RNA was quite low in fractions from infected cells, and because its size is smaller than the T4 and host RNAs, it was not detected by gel blot analysis. The different T4 RNA species present throughout the cell cycle can be readily visualized on northern blots by denaturing polyacrylamide gel electrophoresis.The Trump administration has reportedly struck a deal with Chinese telecom giant ZTE, which will see the US rescind the ban on US companies selling technology to the firm.
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