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A rare case of a young patient with severe hypophosphataemia and hypocalcaemia after high-dose oral steroid therapy is described. Serum levels of 25-hydroxy vitamin D3 were within the normal range, whereas urinary phosphate excretion was increased to 10-fold normal values. This may have been due to osteomalacia. Bone biopsy showed extensive osteomalacia. Corticosteroid therapy may have played a role in the pathogenesis of the osteomalacia. There was no evidence of renal failure or parathyroid dysfunction. After the initiation of phosphate supplementation, hypophosphataemia and hypocalcaemia improved promptly, and the patient recovered from the clinical signs and symptoms.Open Science CRISPR-Cas9: Could it kill a giant in the game of plasmid metapopulations?
CRISPR-Cas9, a powerful genome-editing system, has quickly became the most efficient tool to study all areas of Biology, including the mechanisms of evolution. However, in many cases, its powerful potential could be off-balance by a lack of accurate protocols and methodologies. Here, we show that one of the best-known CRISPR methodologies-plasmid metapopulations (PMs)-is susceptible to a new development of the field: the survival of the fittest-killer
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