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Now it has a research and the resulting ppt papers support the analysis by using the statistical software (Research tools).. Faronics-Anti-Executable-Standard-5.30.1112.606-(x86-x64) Serial-Key~~ 69.) and application of the maximum likelihood method \[[@B13]\]. Haplotypes were reconstructed by PHASE software (version 2.1) using default parameters \[[@B14]\]. The minimum allele frequency (MAF) of the minor alleles was \>0.05. No deviation from Hardy-Weinberg equilibrium (HWE) at every locus was observed after Bonferroni correction. The genetic distance (Fst) among the populations was calculated using Arlequin software (version 3.5) \[[@B15]\]. If Fst values between the areas was high (Fst \> 0.025), we used the genetic program STRUCTURE 2.0 \[[@B16]\] to analyze whether there are four genetic clusters for the local populations, which were based on the co-ancestry coefficient (Q) and the posterior probability (L(K)) using the given preset parameters (K = 4 and LOCPRIOR = 1) \[[@B17]\].
Linkage disequilibrium (LD) analysis and definition of haplotype blocks
———————————————————————–
The levels of LD between pairs of loci were calculated using SHEsis software, which determines the LD value (D’ and r2) and allele frequencies \[[@B18]\]. The haplotype blocks were defined with the method of Gabriel et al. \[[@B19]\]. The level of haplotype blocks was determined by the described set of SNPs: \0.8, very strong haplotype block \[[@B19]\]. All polymorphisms and haplotypes were checked for deviation from HWE.
Population stratification, admixture analysis and gene flow analysis
——————————————————————-
To further detect the genetic differences in the populations and the extent of admixture among populations, model-based STRUCTURE software (version 2.3.1) was used to estimate the population strat
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