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Dietary iron appears to enhance mucosal injury in experimental gastritis. We hypothesized that an iron chelator (3-hydroxypyrid-4-one, 3-OHP) might attenuate gastric damage in an experimental model of chronic gastritis. Male Wistar rats were given H. pylori inoculum and 2 wk later were treated with either normal saline or 3-OHP (75 mg/kg/day) in the drinking water. Rats were sacrificed at 3, 5, and 7 wk and the gastric mucosa and histology of the fundus were examined. Iron staining of tissue sections was performed for iron quantitation. Staining and quantitation was also performed for macrophages, myeloperoxidase, and albumin. The production of prostaglandin E2 (PGE2) was also determined. A highly significant induction of damage in the fundus mucosa of H. pylori-infected animals was seen at all time points studied. The degree of inflammation increased with time. H. pylori infection resulted in marked iron staining in the gastric mucosa; however, the level of iron staining in the 3-OHP-treated group was significantly lower than that in the H. pylori-infected group. Myeloperoxidase and PGE2 levels were significantly lower in the H. pylori/3-OHP group compared with the H. pylori alone group. This study demonstrated that 3-OH
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