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The aim of this study was to investigate whether apoptosis in peripheral blood lymphocytes is involved in the pathogenesis of viral myocarditis. The involvement of apoptosis in the pathogenesis of viral myocarditis was observed in the right and left ventricular myocardium of mice infected with Coxsackievirus B3 strain 15 (CVB3-15) (12 mice), which is associated with clinical disease that closely mimics that observed in humans. Although the spontaneous lymphocyte apoptosis of CVB3-15-infected mice was markedly increased on day 3 postinfection (p.i.), this increase was not statistically significant. However, the apoptosis rate of lymphocytes from heart tissues and serum were significantly increased on day 6 p.i. as compared with that of control mice. The virus-specific T-cell response was observed in the heart by in situ hybridization and enzyme-linked immunosorbent assay. This demonstrates that the apoptosis rate of lymphocytes from heart tissues and serum is increased in association with T-cell response. This is the first evidence that increased apoptosis of peripheral lymphocytes may play a role in the pathogenesis of viral myocarditis.Caminus CP-70 SODAS, CP-80 SODAS, CP-90
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