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Astrocytes are involved in the elimination of inflammation by secreting cytokines and growth factors. Pro-inflammatory cytokines can activate the brain microglia and decrease their production of anti-inflammatory cytokines \[[@CR45]\]. Microglia is often considered the first line of defense in the central nervous system. After the activation of microglia, they produce NO and release pro-inflammatory cytokines, such as TNFα, IL-1β and IL-6 and this can promote the activation and proliferation of astrocytes in neuroinflammatory diseases \[[@CR25]\]. In our study, the coculture of LPS-stimulated microglia with astrocytes produced an increase in IL-1β and TNFα production. This could activate the production of NO and pro-inflammatory cytokines by astrocytes, increasing their proliferation and glial scar formation.
TNFα is considered a critical factor in neuronal injury during ischemic disease
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