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This study was conducted to assess whether the prophylactic infusion of the drug caffeine and the adenosine receptor antagonist pentoxifylline could improve ischemic tolerance. Eighteen anesthetized dogs underwent 35 minutes of coronary artery occlusion followed by 4 hours of reperfusion and were randomly allocated to one of the following groups (n = 6 each): control, caffeine, pentoxifylline, and combined caffeine and pentoxifylline. Infusion of the drugs began immediately before ischemia and was maintained through the reperfusion period. Myocardial blood flow and area at risk were determined. Functional recovery after reperfusion was determined from the recovery of myocardial contractility. The myocardial lactate dehydrogenase concentration was determined as an index of the extent of tissue injury. Myocardial blood flow during ischemia, however, was not different between the groups. At the end of the reperfusion period, the control dogs showed a significant decline in myocardial blood flow and a 50% reduction in area at risk compared with before ischemia. In the caffeine group, myocardial blood flow during reperfusion was maintained at 70% of the values measured before ischemia, and area at risk was preserved at 60% of the original values. The myocardial lactate dehydrogenase content was similar between the groups, and both drugs significantly ameliorated the decrease in area at risk and the functional recovery. We conclude that caffeine and pentoxifylline have no protective effect on ischemic tolerance despite maintaining myocardial blood flow during the reperfusion period, and that the combination of the two drugs results in a significant recovery of ischemic tolerance and a reduced area at risk.Q:
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Factors influencing the enantiomeric ratios of phenylpropionic acids.
Ratios of the enantiomers of phenylpropionic acids were determined by normal-phase HPLC and by capillary electrophoresis using HPLC as an initial analytical technique. The influence of several factors on the enantiomeric ratios of phenylpropionic acids was examined. Acetonitrile addition to the aqueous mobile phase of the normal-phase HPLC caused major alterations of the enantiomeric ratios, whereas pre-column derivatisation of phenylpropionic acids, using chiral reagents, improved the quality of the analytical results. Addition of acetic acid to the chromatographic eluent is proposed as an alternative to the addition of acetonitrile. The enantiomeric ratios of 20 phenylpropionic acids were determined as a function of the most important factors affecting the quality of the enantiomeric ratios of phenylpropionic acids: acetonitrile content of the eluent and acetic acid content of the eluent. It is shown that ratios in the range 1.5-12 can be reached. In order to determine the enantiomeric ratios in the range 0.6-0.94, the impact of the enantiomeric ratios on the size of the resolution factor R for two enantiomeric pairs cannot be neglected.The University of Cape Town (UCT) has a comprehensive animal welfare programme for its University of Life Sciences and Health Studies (ULSH) Animal Resources. ULSH has been investigating and conducting research with animals for many years, especially in the field of veterinary medicine and clinical medicine. Currently, 9 of
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