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β-Casomorphins are peptide fragments of β-casein, which can be released from bovine milk after hydrolysis by endogenous, mammalian enzymes or by bacterial proteases. Many studies have shown that β-casomorphins are neuroactive and can regulate gastrointestinal function. However, there is little information on the effects of β-casomorphins on inflammation. The aim of this study was to examine the effects of β-casomorphins on mouse mast cell inflammatory responses in vivo. A β-casomorphins-5 liposomal suspension (≥300 µg/ml) was administered intraperitoneally into BALB/c mice, and the effects of β-casomorphins-5 were investigated by measuring both blood and peritoneal lavage fluid parameters. Mast cell activation in various tissues was assessed by toluidine blue staining. β-Casomorphins-5 significantly reduced mouse blood urea nitrogen levels compared with vehicle controls after 30 min post-administration. β-Casomorphins-5 significantly decreased both the number and activation of peritoneal mast cells. Furthermore, β-casomorphins-5 suppressed mouse mast cell accumulation in four different tissues, such as the peritoneal cavity, lung, heart,
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