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(This is the second half of the article). How to Install SQL Server Express With Database That Does Not Have Service Pack. You might have already heard about SQL Server Express Edition.
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We developed a pipeline for TMB estimation by coupling the information from RNA-seq reads with the gene structure from the genome. The exon number calculation based on RNA-seq data was implemented by calculating the total number of reads mapped to a gene based on the percentage of exonic base coverage. For each gene, we calculated the gene size by applying the formula gene size = 100\*(the sum of all exonic base covered by the reads mapped to the gene)/the number of mapped reads. To test the accuracy of this method, we used three independent RNA-seq datasets from different tissues and calculated the correlation between gene size and read count.
Gene expression estimation {#Sec14}
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We obtained the FPKM value of protein coding genes in each dataset by the Cufflinks software (v2.2.0).
Identification of alternatively spliced genes {#
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