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The thermal unfolding of cytochrome c peroxidase (CcP) has been followed by circular dichroism (CD), UV-CD and dynamic light scattering (DLS) to determine the role of structure in the loss of function upon thermal denaturation. The unfolding transition is described by a three-state model where the native state is largely unfolded (40%), whereas the intermediate and final state are virtually structureless and fully unfolded. At a first glance, this is quite similar to the unfolding behavior of the highly homologous model protein, a monomeric azo-initiator horseradish peroxidase. However, it is shown here, by fluorescence spectroscopy, that the native protein and the fully unfolded protein are spectrally identical. The rate of unfolding is extremely slow (k2 approximately 0.07 degrees C-1) compared to the widely used model proteins. The results are not compatible with a dehydration mechanism since this would involve the loss of a large number of partially folded states upon unfolding. The implications of this scenario are discussed.So even for the most seasoned of adult fans, watching Cameron Diaz in “The Counselor” she’s playing should be like catching up on tips on how to get a baby out of a watermelon without a knife. It’s not that she’s having flashbacks to her teen years while trying to hold it in. It’s that the we see so little of her character that we don’t know what she’s actually like. In one scene, she’s wearing a dress that makes her look like she got dressed in the Twilight Sparkle costume aisle at Party City, but whenever the character we see her in a sexual situation with tries to nuzzle her face, the action is so quickly captured that we couldn’t tell you whether that was a kiss or a groping, which is the kind of ambiguity that created so many not-nice comments after the “American Pie” movies that weren’t about sex but somehow involved sex. This is the first Diaz has played a character of this type for a number of years, with “American Wedding�

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