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Printing is a hard business. We all know that because we see the painfully high printing costs on just about every product we purchase. As a result, printing in the Web isn’t something we usually enjoy doing. Thankfully, technology is making printing smaller, more affordable, and allowing you to create even more products and more variations than you could have dreamed of. However, as amazing as the Web is, printing can still be done in one of the most effective and cost-efficient ways possible: printing from your computer. That’s right, while you’re surfing to and from a Web site, printing various documents from the comfort of your desk, or all the while you’re at the offices or at home, you can have the ability to print the products and services you’re looking for. Moreover, printing from your computer isn’t only possible for the home or office user. From a small store kiosk, through regional office desks, to the next-generation e-mail printer, this ability to print from a computer can be extremely important for those responsible with today’s communications. It will be a requirement to print any business or personal documents from your computers — without the Web, you’ll be left without the ability to do so. In addition, if you don’t have a compatible printer or if you want to add a feature (such as a wireless printing option) to your existing printer, it can be expensive. The printer will not be able to support these functions if it’s not compatible with your computer. As a result, it’s always beneficial to know how you can print from your computer so you can ensure you can print any document from anywhere you are, when you need it. Uncovering the fun in print
The print process is when documents can come off of a computer. This can include text documents, pictures, brochures, logos, and marketing materials. Because the process is essentially taking an electronic document and printing it to something physical, it allows for people to experience the product in a way they couldn’t before. So when you create a printed document for your business, you are creating for yourself a unique marketing piece. In addition, another advantage of print is it’s saving trees. Printing is the most sustainable form of publication; this is because it doesn’t require as much energy to create or use as other modes.
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This study indicates that estrogen-responsive luciferase reporter gene constructs in CV-1 cells lack the robust and consistent responses seen in MCF-7 human breast cancer cells. When these cells were transfected with an estrogen-responsive luciferase reporter construct, the observed luciferase response was about fivefold higher than that seen in CV-1 cells. This difference could be caused by differential expression of the G protein involved in coupling estrogen receptor to the cell membrane. To compare estrogen receptor expression across cell lines and between human tissues, we analyzed estrogen-responsive luciferase activity in a panel of several human cell lines, including CV-1, MCF-7, and HeLa cells. These cell lines express similar amounts of estrogen receptor, but exhibit a range of luciferase responses when treated with either 2.5 nM (MCF-7) or 10 nM (CV-1) 17beta-estradiol. Compared to these cell lines, transfection of HeLa cells with a luciferase reporter gene construct elicited a 10-fold increase in luciferase activity following 2.5 nM 17beta-estradiol treatment. To determine whether the relative inducibility of luciferase observed in CV-1 cells and HeLa cells was due to species differences in estrogen receptor expression, the cellular localization of the estrogen receptor
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