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composition and sensory quality of the oil from xanthophyll-rich Hibiscus sabdariffa calyces: an Arabidopsis thaliana thiamine thiophosphate deficient mutant as an ex situ model of human abetalipoproteinemia.
Hibiscus sabdariffa L. (Malvaceae) is widely grown as a vegetable and used as a berry. In this study, we aimed to investigate the nutritional value and sensory quality of the oil from calyx, in comparison to that from leaves. The calyces and leaves were hydrolyzed in 5% KOH for 1 h at 100°C to evaluate total phenolic and flavonoid content. It was shown that calyces are characterized by higher concentration of total polyphenols (37.0 and 65.1 mg CAE/g DW), total flavonoids (80.7 and 166.1 mg QE/g DW) and vitamin C (230.4 and 268.3 mg L-ascorbic acid/g DW) and higher antioxidant activity index (78.3 and 106.6 mM TE/100 g DW), than leaves. Scanning electron microscopy and X-ray diffraction indicated that the oil obtained from calyces is characterized by high lipid content (73.9%), higher melting point (60°C) and higher solid fat content than that obtained from leaves (53.5%, 46°C and 15%). The presence of xanthophylls was confirmed by high-performance liquid chromatography. This study showed that although calyx oil has a higher concentration of phytochemicals, it is less than desirable for this specific target market. Arabidopsis thaliana plants, showing perturbed thiamine metabolism due to the loss of thiamine thiophosphate in the ascorbate biosynthesis pathway, were characterized in terms of viability, phenolic content and sensory quality of their leaves and calyces. The
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